Next-Generation Sequencing-Based Decision Support for Intensivists in Difficult-to-Diagnose Disease States: A Case Report of Invasive Cerebral Aspergillosis
Aspergillus spp. are widespread environmental pathogens that can induce invasive aspergillosis, especially in immunocompromised patients.
Fast identification of disseminated mucormycosis in hematooncologic patients by next-generation sequencing: a case series.
Invasive mucormycoses are increasingly emerging and a severe health threat with potentially fatal outcome especially for immunocompromised patients. They are challenging to diagnose: the diagnostic workup includes numerous tests, is time-intensive and frequently invasive. Cultivation is difficult and slow and yet frequently remains negative. Fast diagnosis for a timely treatment is urgently needed. We here describe a case series, where untargeted next-generation sequencing (NGS, with Noscendo’s CE-IVD marked DISQVER® pathogen test) provided identification of moulds in three patients with underlying hematologic malignancies from a standard blood draw within 24h after sample arrival.
Pathogen detection by metagenomic next-generation sequencing during neutropenic fever in patients with haematological malignancies
Febrile neutropenia (FN) following chemotherapy is a major cause of morbidity requiring urgent use of broad-spectrum antibiotics. Blood culture (BC) is a standard diagnostic method for FN but sensitivity of pathogen detection is low. Timely identification of causative pathogens using metagenomic next-generation sequencing (mNGS) of circulating cell-free DNA (cfDNA) from plasma could reduce morbidity and costs by enabling early-targeted antimicrobial therapy. The aim of this study is to establish mNGS (DISQVER) for the detection of pathogens within 24h in immunocompromised haematological patients with FN
Immune-Response Patterns and Next Generation Sequencing Diagnostics for the Detection of Mycoses in Patients with Septic Shock—Results of a Combined Clinical and Experimental Investigation
Int. J. Mol. Sci. 2017 August. doi: 10.3390/ijms18081796
J MOL DIAGN 2020 March. doi: https://doi.org/10.1016/j.jmoldx.2019.12.006 Learn...
Enhanced Performance of Next-Generation Sequencing Diagnostics Compared With Standard of Care Microbiological Diagnostics in Patients Suffering From Septic Shock
Crit. Care Med. 2019 May. doi: 10.1097/CCM.0000000000003658
New approaches for the detection of invasive fungal diseases in patients following liver transplantation—results of an observational clinical pilot study
Langenbecks Arch. Surg. 2019 May. doi: 10.1007/s00423-019-01769-y
JGenome Med. 2016 Dezember. doi: 10.1186/s13073-016-0326-8
Next-generation sequencing diagnostics of bacteremia in sepsis (Next GeneSiS-Trial): Study protocol of a prospective, observational, noninterventional, multicenter, clinical trial
Medicine (Baltimore) 2018 February. doi: 10.1097/MD.0000000000009868